Products > PM Systems > Standard PM System
Complete Turnkey System
The InCyt Standard PmTM Imaging System includes everything needed for calcium and other single excitation and ratiometric fluorophore experiments. The system includes: an inverted epi-fluorescence/phase contrast microscope, low-light level integrating CCD camera, computer controlled filter changer, Xenon UV/VIS arc lamp, image processing computer and data acquisition/analysis software. |
The InCyt Standard PmTM Imaging System was designed in a biomedical research laboratory by scientists for scientists. Menu selection appears in the sequence in which experiments are performed. Esoteric options that add complexity and are rarely used have been eliminated from the menus. The software follows standard Windows graphical-user-interface-protocol. The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation. New users can learn to use the system and be doing their first experiment in a few hours. |
Data Collection, Analysis & Presentation
Data can be measured continuously on as many as 50 pre-selected regions of interest. The results can displayed in real time or images can be saved for post hoc analysis. Saved images can be played back as an animated sequence—ideal for analysis of sub-cellular responses, intercellular communication and heterogeneity among the individual cells in a population. Ion concentration within cells can be converted to color using standard or custom pseudo-color tables. Image sequences can be presented as a montage with user-defined annotation for presentation and publication. Data generated by InCyt Standard PmTM are stored in a standard TIFF format and data files are stored as ASCII text. |
Photometer Module |
|
| PMT | Hamamatsu HC135-11 photon counter |
| Linearity (precision) | +/-1% from 0 to 20,000,000 counts/second |
| Maximum Dark Count (noise) | 150 counts/second |
| Equivalent Noise Input (noise) | 3 x 10-17 watts per second (measured at 400nm) |
| Spectral Sensitivity | 300-650nm |
| Discriminator & Microprocessor | Built into PMT |
| Overload Protection | Computer-controlled shutter (optional) |
| Excitation Light Source | Sutter Instruments LB-LS17 light source with 175W Xenon arc lamp for both UV and visible light excitation. |
| Filter Changer | Tofra Wheel Filter Changer |
| Data Acquisition and Data Analysis Workstation | |
| CPU | Intel® 2nd generation Core™ i3 Dual Core Processor |
| RAM | 4GB Non-ECC dual-channel 1000MHz DDR3 SDRAM |
| Hard Drives | 250GB SATA 6.0Gb/s and 8MB DataBurst Cache |
| DVD ROM | 16X DVD +/- R/W |
| Operating System | Windows 7 |
| Monitor | 20" |
| Data Capture and Display | |
| Maximum Speed | 6 ratiometric points per second 100 single-wavelength points per second |
| Time Lapse | Up to 1 measurement every 10 minutes |
| Field of View | Rectangular area, user-definable |
| Graphic Display | Real-time during experiment |
| Data Storage Format | Tab-delimited ASCII |
| Other | |
| Calibration | From standard solutions or formula, or in situ |
| Ions | Works with hundreds of single-excitation, single-emission fluorescent dyes |