The InCyt-Pm line of photometry systems utilize super-sensitive photomultipliers to measure changes in fluorescence. The advantages over imaging are dramatically increased speed of data aquisition and light sensitivity. The disadvantage is that the entire field of view is treated as a single object at each time point. Mitigating this problem is that the size of the field view can be varied. |
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Basic PM >> |
Standard PM >> |
High Speed PM >> |
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| Speed of data acquisition |
6 ratiometric points per second 100 single-wavelength points per second |
6 ratiometric points per second 100 single-wavelength points per second |
150 ratiometric measurements per second |
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| Photometer | 1 msec time resolution |
1 msec time resolution |
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| Microscope | Accuscope 3032 Inverted Fluorescence Microscope |
Nikon TS100F Inverted Fluorescence Microscope |
Nikon TS100F Inverted Fluorescence Microscope |
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| Filter changer | Tofra Wheel Filter Changer | Sutter Wheel Filter Changer | Sutter DG5 fast Wavelength Changer with built-in Xenon arc lamp | |||
| Light source | Sutter LB-LS17 175 watt Xenon arc lamp for both UV and visible light excitation. | Sutter LB-LS17 175 watt Xenon arc lamp for both UV and visible light excitation. |