The InCyt-Pm line of photometry systems utilize super-sensitive photomultipliers to measure changes in fluorescence. The advantages over imaging are dramatically increased speed of data aquisition and light sensitivity. The disadvantage is that the entire field of view is treated as a single object at each time point. Mitigating this problem is that the size of the field view can be varied. |
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| -- | Standard PM >> |
High Speed PM >> |
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| Maximum Maximum speed of data acquisition | -- | 6 ratiometric points per second 100 single-wavelength points per second |
150 ratiometric measurements per second |
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| Photometer | -- | 1 msec time resolution |
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| Microscope | -- | Olympus CKX53 Inverted Fluorescence Microscope |
Olympus CKX53 Inverted Fluorescence Microscope |
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| Filter changer | -- | Sutter Wheel Filter Changer | CoolLED pE-340fura LED light source with 340nm & 380nm LEDs and a white LED which can be filtered for visible light excitation | |||
| Light source | -- | Sutter LB-LS17 175 watt Xenon arc lamp for both UV and visible light excitation. |