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Complete Turnkey System

The InCyt Basic I/P TM Imaging System includes everything needed for calcium and other single excitation and ratiometric fluorophore experiments. The system includes: an inverted epi-fluorescence/phase contrast microscope, low-light level integrating CCD camera, computer controlled filter changer, Xenon UV/VIS arc lamp, image processing computer and data acquisition/analysis software.

Ease-of-use

The InCyt Basic I/P TM Imaging System was designed in a biomedical research laboratory by scientists for scientists. Menu selection appears in the sequence in which experiments are performed. Esoteric options that add complexity and are rarely used have been eliminated from the menus. The software follows standard Windows graphical-user-interface-protocol. The hardware has been tested for reliability and ruggedness, as well as, simplicity of set up and operation. New users can learn to use the system and be doing their first experiment in a few hours.

Data Collection, Analysis & Presentation

Data can be measured continuously on as many as 50 pre-selected regions of interest. The results can displayed in real time or images can be saved for post hoc analysis. Saved images can be played back as an animated sequence—ideal for analysis of sub-cellular responses, intercellular communication and heterogeneity among the individual cells in a population. Ion concentration within cells can be converted to color using standard or custom pseudo-color tables. Image sequences can be presented as a montage with user-defined annotation for presentation and publication. Data generated by InCyt Basic I/P TM are stored in a standard TIFF format and data files are stored as ASCII text.

Specifications

Camera Module
Camera

Basler Scout Monochrome Integrating 12-bit CCD
Excitation Light Source

Sutter Instruments LB-LS17 light source with 175W Xenon arc
lamp for both UV and visible light excitation.

Filter Changer Tofra Wheel Filter Changer
Image Resolution 640 x 480 pixels

Camera Module
PMT

Hamamatsu HC135-11 photon counter
Linearity (precision)

+/-1% from 0 to 20,000,000 counts/second
Maximum Dark Count (noise) 150 counts/second
Equivalent Noise Input (noise) 3 x 10-17 watts per second (measured at 400nm)
Spectral Sensitivity 300-650nm
Discriminator & Microprocessor Built into PMT
Overload Protection Computer-controlled shutter (optional)
Excitation Sutter Instruments LB-LS17 light source with 175W Xenon arc lamp for both UV and visible light excitation.
Filter Changer Tofra Wheel Filter Changer

Data Acquisition and Data Analysis Workstation
CPU

Intel® 2nd generation Core™ i3 Dual Core Processor

RAM 4GB Non-ECC dual-channel 1000MHz DDR3 SDRAM
Hard Drives 250GB and 1TB 3.5" SATA 6Gb/s with 8MB DataBurst Cache
DVD ROM 16X DVD +/- R/W
Operating System Windows 7
Monitor 20" SVGA

Data Capture and Display
Maximum Speed 1.5 ratiometric points per second
15 single-wavelength points per second
Object Definition Specify up to 50 user-defined regions for separate analysis
Data Collection Options (1) Graph ion concentration in each cell during experiment.
(2) Save images for later analysis and animated playback
Image Forma TIFF
Data Storage Format Tab-delimited ASCII

Other
Calibration From standard solutions or formula
Ions

Works with hundreds of fluorescent dyes for measuring:

Ca2+, pH, Na+, K+, Mg+2, H2O2, cyclic ADP Ribose, etc.




Data Presentation